Development of ion-sequensing method using boronic acid

2016 Fiscal Year Final Research Report

• Project/Area Number: 26702013
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Matsumoto Akira 東京医科歯科大学, 生体材料工学研究所, 准教授 (70436541)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ボロン酸 / ピロリン酸 / 核酸増幅

Outline of Final Research Achievements

We found that a heterocyclic class of boronic acids, based on the pyridine structures, can interact with pyrophosphate and some of its derivatives in a specific, reversible and pH-dependent manner. Based on this finding, we prepared a series of boronate-modified electrodes and confirmed their sensitivity to pyrophosphate with the detection limit of 30 uM. Moreover, we designed a polymeric material that is able to undergo a sharp inversion in the state of hydration in synchronization with recognition of pyrophosphate. In combination with a common thermal cycle protocol in nucleotide amplification reactions, our finding may help dramatically simplify those ongoing technologies.

Free Research Field

生体計測、ドラッグデリバリーシステム