2015 Fiscal Year Final Research Report
Production of a structurally and functionally biomimetic 3D muscle tissue construct using cell sheet-based tissue engineering
| Project/Area Number |
26702018
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 組織工学 / 再生医療 / 細胞シート / 骨格筋 |
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| Outline of Final Research Achievements |
In mature skeletal muscle, the muscle fibers are highly oriented to produce its mechanical functions, and vasculature and neurons also have well-organized structures. Using a patterned thermoresponsive culture substrate, we produced a cell sheet composed of aligned skeletal muscle myoblasts. This cell sheet was attached on a 3D gel and then incubated in differentiation medium. As a result, contractile muscle tissue construct was successfully produced. The muscle contraction was observed by electrical stimulation, and it was suppressed by the addition of some kinds of ion channel blockers. In addition, human neurons and human endothelial cells were incorporated within the multilayer cell sheets for creating a complex tissue construct. Both type of cells formed oriented networks guided by the myoblast orientation. The structural design and sequential functionalization in future work could lead to truly biomimetic tissue generation, and development of in-vitro physiological tissue models.
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| Free Research Field |
組織工学・バイオマテリアル
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