Elucidation of sleep-wake mechanism by controlling cell function

2016 Fiscal Year Final Research Report

• Project/Area Number: 26702037
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Basic / Social brain science
• Research Institution: Toho University (2016); Tokyo Metropolitan Institute of Medical Science (2014-2015)
• Principal Investigator: UENO Taro 東邦大学, 理学部, 講師 (30648267)
• Co-Investigator(Renkei-kenkyūsha): FURUTA Toshiaki 東邦大学, 理学部, 教授 (90231571)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 睡眠 / ケミカルジェネティクス

Outline of Final Research Achievements

Fluorescein-modified compounds were prepared and added to the transfected cells, and a marked fluorescence enhancement of fluorescein was observed in cells expressing PLE.
We generated a transgenic fly expressing PLE only in specific cells in the expression-dependent manner of GAL4, and when the above compound was administered to the isolated brain, tissue-specific fluorescence was observed.
In order to examine whether behavior control at an individual level is possible, Drosophila expressing PLE specifically for dopamine cells was created. A compound to which a modifying group was added to a dopamine synthase inhibitor was administered by oral administration, but no change in sleep awakening amount was observed.

Free Research Field

睡眠科学