2016 Fiscal Year Final Research Report
Elucidation of sleep-wake mechanism by controlling cell function
Project/Area Number |
26702037
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Partial Multi-year Fund |
Research Field |
Basic / Social brain science
|
Research Institution | Toho University (2016) Tokyo Metropolitan Institute of Medical Science (2014-2015) |
Principal Investigator |
UENO Taro 東邦大学, 理学部, 講師 (30648267)
|
Co-Investigator(Renkei-kenkyūsha) |
FURUTA Toshiaki 東邦大学, 理学部, 教授 (90231571)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | 睡眠 / ケミカルジェネティクス |
Outline of Final Research Achievements |
Fluorescein-modified compounds were prepared and added to the transfected cells, and a marked fluorescence enhancement of fluorescein was observed in cells expressing PLE. We generated a transgenic fly expressing PLE only in specific cells in the expression-dependent manner of GAL4, and when the above compound was administered to the isolated brain, tissue-specific fluorescence was observed. In order to examine whether behavior control at an individual level is possible, Drosophila expressing PLE specifically for dopamine cells was created. A compound to which a modifying group was added to a dopamine synthase inhibitor was administered by oral administration, but no change in sleep awakening amount was observed.
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Free Research Field |
睡眠科学
|