Development of Digital Nanochannels for Single Cell Omics

2016 Fiscal Year Final Research Report

• Project/Area Number: 26706010
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Nano/Microsystems
• Research Institution: Osaka Prefecture University
• Principal Investigator: Xu Yan 大阪府立大学, 工学(系)研究科(研究院), 准教授 (90593898)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: デジタル化ナノチャネル / 単一細胞オミクス / 細胞計測 / ハイスループット計測 / Nano-in-Nano集積化 / ナノ流体デバイス / １分子計測

Outline of Final Research Achievements

In this study, we established technology base for the proof-of-concept of a new technology called digital nanochannel (DN), which was proposed by us and holds great potential allowing single cell omics analysis at the single molecule level. First, the basic technology for fabricating nanofluidic chips with precise, high density, stable DN structures was developed. Second, the mechanism of signal detection for single molecule counting by DN was confirmed. Third, fundamental technologies to improve the performance of single molecule detection by DN, such as suppression of nonspecific protein adsorption in DN and regeneration of DN chips, were further established. These results promise that DN could be a powerful tool for single cell omics studies after further development in the future.

Free Research Field

ナノ化学システム、分析化学、バイオマテリアル