Growth inhibition of Pseudomonas aeruginosa by HasA with metal-phthalocyanine and a novel elimination system of bacteria by photo-irradiation

2017 Fiscal Year Final Research Report

• Project/Area Number: 26708018
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Nagoya University
• Principal Investigator: Shoji Osami 名古屋大学, 理学研究科, 准教授 (90379587)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: ヘム / 緑膿菌 / 増殖阻害 / フタロシアニン / ポルフィリン / 鉄 / 光照射 / 一重項酸素

Outline of Final Research Achievements

HasA (Heme acquisition system A) is a hemophore secreted by some pathogenic bacteria having a heme acquisition system (Has system) such as Pseudomonas aeruginosa. We have found that HasA can capture several synthetic metal complexes other than heme such as iron-salophen and iron-phthalocyanine. The crystal structures of HasA harboring iron-salophen and iron-phthalocyanine showed only small structural perturbations compared with that of HasA with heme. We also found that HasA bound to iron-phthalocyanine strongly inhibited HasA-mediated heme acquisition. Furthermore, we have demonstrated that Pseudomonas aeruginosa can be eliminated by photo-irradiation at 680 nm in the presence of HasA bound to gallium-phthalocyanine due to generation of singlet oxygen.

Free Research Field

生物無機化学