2017 Fiscal Year Final Research Report
Growth inhibition of Pseudomonas aeruginosa by HasA with metal-phthalocyanine and a novel elimination system of bacteria by photo-irradiation
Project/Area Number |
26708018
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Partial Multi-year Fund |
Research Field |
Bio-related chemistry
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Research Institution | Nagoya University |
Principal Investigator |
Shoji Osami 名古屋大学, 理学研究科, 准教授 (90379587)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Keywords | ヘム / 緑膿菌 / 増殖阻害 / フタロシアニン / ポルフィリン / 鉄 / 光照射 / 一重項酸素 |
Outline of Final Research Achievements |
HasA (Heme acquisition system A) is a hemophore secreted by some pathogenic bacteria having a heme acquisition system (Has system) such as Pseudomonas aeruginosa. We have found that HasA can capture several synthetic metal complexes other than heme such as iron-salophen and iron-phthalocyanine. The crystal structures of HasA harboring iron-salophen and iron-phthalocyanine showed only small structural perturbations compared with that of HasA with heme. We also found that HasA bound to iron-phthalocyanine strongly inhibited HasA-mediated heme acquisition. Furthermore, we have demonstrated that Pseudomonas aeruginosa can be eliminated by photo-irradiation at 680 nm in the presence of HasA bound to gallium-phthalocyanine due to generation of singlet oxygen.
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Free Research Field |
生物無機化学
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