in cell Templated Reaction by employing in vitro selected RNA aptamers

2015 Fiscal Year Final Research Report

• Project/Area Number: 26708020
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: UZAWA Takanori 国立研究開発法人理化学研究所, 伊藤ナノ医工学研究室, 専任研究員 (60554376)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: アプタマー / ルテニウム錯体

Outline of Final Research Achievements

I aim to construct a method to determine position and concentration of a specific mRNA in a live cell. We tried to select and utilize the aptamers for two compounds: ruthenium trisbipyridine whose photo-exited state possesses strong redox ability and aziderhodamine which emits fluorescence after reduction to amino rhodamine by the photo-exited ruthenium trisbipridine. We have found that the aptamer which binds ruthenium trisbipyridine elongated the lifetime of the photo-exited ruthenium tirsbipyridine 4 fold by reducing oxygen quenching. Although we have tried 30 candidate RNA obtaind by the selection for aziderhodamine, none of them exhibit a reproducible positive result.

Free Research Field

進化分子工学