2016 Fiscal Year Final Research Report
Light-assisted liquid chromatography of proteins using carbon nanotubes
| Project/Area Number |
26708021
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Hirano Atsushi 国立研究開発法人産業技術総合研究所, ナノ材料研究部門, 主任研究員 (90613547)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ナノチューブ / 蛋白質 |
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| Outline of Final Research Achievements |
A liquid chromatography column using carbon nanotubes as ligands was fabricated for realizing light-assisted liquid chromatography for protein separation. It was found that the carbon nanotubes immobilized in the column interact with aromatic amino acids, especially with tryptophan, and that they oxidize cysteine, resulting in disulfide formation. Thus, prevention of excess protein adsorption onto the carbon nanotubes and suppression of cysteine oxidation are necessary for fine control of the protein separation in this system.
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| Free Research Field |
蛋白質科学
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