Immunogenetic Repertoire Analysis for Tumor-infiltrating Lymphocytes - toward the Understanding of Tumor Immunology -

2016 Fiscal Year Final Research Report

• Project/Area Number: 26710009
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Tumor therapeutics
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Katoh Hiroto 東京医科歯科大学, 難治疾患研究所, 助教 (60446549)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 免疫ゲノム / ゲノム解析 / 腫瘍免疫

Outline of Final Research Achievements

The purpose of this study was to comprehensively analyze the antigen receptor repertoires of tumor-infiltrating lymphocytes by the next generation sequencing and to elucidate the global picture of tumor immunity from the viewpoint of immuno-genetics. Additionally, I also aimed to create a basic database to develop new therapeutic strategies against cancers, such as novel anti-cancer antibodies, based on antigen receptor repertoire sequences that existed dominantly in cancer tissues. The following achievements were obtained.
(1) Next generation sequencing protocols for the antigen receptor repertoires of tumor-infiltrating lymphocytes in clinical cancer tissues were established. (2) A number of T cell receptors and immunoglobulins that were specifically and dominantly infiltrated in cancer tissues were identified. (3) Functional screening of multiple cancer specific immunoglobulin molecules reconstructed by artificial expression system could be carried out.

Free Research Field

ゲノム病理学