Epigenetic mechanism about immediate early transcribed genes in vascular endothelial cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26710013
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Medical genome science
• Research Institution: The University of Tokyo
• Principal Investigator: KANKI YASUHARU 東京大学, アイソトープ総合センター, 助教 (00534869)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 血管新生 / エピゲノム / VEGF / ChIP-seq / ヒストン修飾 / 抗がん剤

Outline of Final Research Achievements

In solid tumor, cancer cells secrete vascular endothelial cell growth factor (VEGF) by themselves in order to get oxygen and nutrition. In this report, our aim is to elucidate a novel epigenetic mechanism about immediate early transcribed genes in vascular endothelial cells under VEGF stimulation.
At first, we performed ChIP-sequence by using various histone modification antibodies including H3K4me3, H3K27me3, H3K27ac, and H2AK119Ub. We clarified that H3K4me3 modification was induced within 15min on EGR3 locus. In addition, the inhibition of H3K4me3 modification by PTIP knockdown caused the reduction of VEGF-induced genes transcription in vitro and in vivo.
Taken together, these findings have suggested that inhibition of PTIP might be useful material for the development of anti-angiogenic drugs in the future.

Free Research Field

血管生物学