Establishment of novel adaptive optics microscopy enabling high-resolution live imaging during stem-cell formation

2017 Fiscal Year Final Research Report

• Project/Area Number: 26711010
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biophysics
• Research Institution: National Institute for Basic Biology
• Principal Investigator: TAMADA Yosuke 基礎生物学研究所, 生物進化研究部門, 助教 (50579290)
• Research Collaborator: HATTORI Masayuki 国立天文台, TMT推進室, 特任助教 (20308208) ; HAYANO Yutaka 国立天文台, 先端技術センター, 准教授 (80390623) ; OYA Shin 国立天文台, TMT推進室, 特任准教授 (80399287) ; MIURA Noriaki 北見工業大学, 工学部, 教授 (30209720) ; KAMEI Yasuhiro 基礎生物学研究所, 生物機能解析センター, 特任准教授 (70372563) ; MURATA Takashi 基礎生物学研究所, 生物進化研究部門, 准教授 (00242024)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 補償光学 / ライブイメージング / 顕微鏡 / 画像相関 / 幹細胞 / 植物 / 光学特性

Outline of Final Research Achievements

Images inside living cells and tissues always degrade because the light used for the imaging is disturbed by biological structures. This project aims at applying adaptive optics (AO) to microscopy to correct the disturbance of light and performing high-resolution deep live imaging during stem-cell formation of the moss Physcomitrella patens. We revealed the disturbance of light that goes through living plant cells by point spread function analysis, mathematical calculation, and simulation. This is essential to establish high-accuracy AO system. In addition, we increased the sensitivity of wavefront sensing around 25 folds, and also obtained the wavefront information by image correlation. These allowed us to perform wavefront sensing and drive AO in most place in living cells and to reduce damages caused by excitation light. Applying above achievements to our AO microscope, we successfully perform fine live imaging inside living plant cells.

Free Research Field

光学、イメージング科学、生物物理学、細胞生物学