Development versatile zygote-mediated genome-editing technologies in mice

2017 Fiscal Year Final Research Report

• Project/Area Number: 26712025
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: The University of Tokyo
• Principal Investigator: Fujii Wataru 東京大学, 大学院農学生命科学研究科(農学部), 助教 (40708161)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 発生工学 / 人工ヌクレアーゼ

Outline of Final Research Achievements

We attempted to establish more versatile system to generate genetically-modified mice by using zygote-mediated engineered endonucleases. We were successfully applied S. thermophiles 1- and C. jejuni-derived CRISPR/Cas to zygote-mediated generation of knockout/knock-in mice. We found that exogenous dsDNA-injection activates ooplasmic ATM signaling pathway and inhibits preimplantation development. By using the established method, we generated several mouse strains which replaced the orthologous locus/loci to porcine or giraffe gene sequence.

Free Research Field

発生工学