Establishment of an efficient maintenance and expansion system for spermatogonial stem cells from wide variety of animals.

2017 Fiscal Year Final Research Report

• Project/Area Number: 26712026
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: Kyushu University
• Principal Investigator: IWAMORI Naoki 九州大学, 大学院農学研究院, 准教授 (70647362)
• Research Collaborator: IWAMORI Tokuko 九州大学, 大学院医学研究院, 助教 (10711509) ; SHIMA Sakurako ; YAMAMURA Yuki
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 精子幹細胞 / 細胞間架橋 / エピジェネティクス / JMJD3

Outline of Final Research Achievements

An efficient culture system for spermatogonial stem cells of animals except for rodent has not been developed, although it is about fifteen years from first establishment of culture system for mouse spermatogonial stem cells. In this study, we have tried to develop an efficient maintenance and expansion for wide variety of animals using fragmentation of spermatogonial cysts by abscission of intercellular bridges which is evolutionarily well conserved from invertebrate to vertebrate. We have generated an spermatogonial stem cells in which fragmentation of spermatogonial cysts is visualized. In addition, molecular mechanisms which could be responsible for abscission of intercellular bridges were analyzed using germ cell specific JMJD3 deficient mice in which fragmentation of spermatogonial cysts was frequently occurred. A prospective molecular mechanisms which could regulate fragmentation of spermatogonial cysts has be identified.

Free Research Field

生殖生物学