2015 Fiscal Year Final Research Report
Mechanism of bacterial homeostasis mediated by transporters and development of new therapeutic strategies to control infectious diseases
| Project/Area Number |
26713004
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | トランスポーター / 薬剤耐性 / サルモネラ / 大腸菌 / 緑膿菌 / 遺伝子発現制御 / バイオフィルム / 構造生物 |
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| Outline of Final Research Achievements |
We found that the bile-mediated activation of the acrAB and tolC multidrug efflux genes occurs mainly through transcriptional derepression of ramA in Salmonella. It was revealed that multidrug efflux pumps contribute to E. coli biofilm maintenance. It was discovered that AcrB, AcrD, and MdtABC multidrug efflux systems are involved in enterobactin export. We also investigated the effect of methylglyoxal on multidrug-resistant P. aeruginosa, and roles of Salmonella multidrug efflux pumps in decreased susceptibility to triclosan. The structures of the regulator bound with bile acids were also solved, and we performed the phenotype microarray analysis of the drug efflux systems in Salmonella enterica serovar Typhimurium.
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| Free Research Field |
生体分子制御科学研究分野
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