Fabrication of 3-dimensional functional cardiac tissue from human iPS cell derived cardiomyocyets for heart regeneration

2017 Fiscal Year Final Research Report

• Project/Area Number: 26713044
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Kumamoto University (2016-2017); Saga University (2014-2015)
• Principal Investigator: Noguchi Ryo 熊本大学, 医学部附属病院, 助教 (70530187)
• Research Collaborator: MUKAE Yosuke 佐賀大学, 医学部心臓血管外科
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 再生医療 / 心筋再生 / 心不全治療 / 組織工学

Outline of Final Research Achievements

This study aimed to fabricate 3-Dimensional functional cardiac tissue from human iPS derived cardiomyocytes which treats human failed heart. We focused on and utilize well known phenomenon spheroid formation of cell. Human iPS cell derived cardiomyocytes, human vascular endothelial cells, fibroblasts, and neural cell were fused to form contractile cardiac spheroids. Firstly, we optimize the cell amount, combination ratio, and another cell source to create better contractile spheroid. We found the addition of iPS cell derived neural cell increase function of cardiac spheroid. Second, we developed 3-Dimensional fabrication system. Utilizing this system, spheroids were easily arranged to fabricate cardiovascular patch construct, and tubular consturact. Combing spheroids phenomenon and tissue engineering devices, these constructs containe enough extracelluar matrix, microvascular networks in the construct.

Free Research Field

心臓血管外科