Characterization and gene expression analysis of enzymes from a water extract of Ganoderma lucidum

2016 Fiscal Year Final Research Report

• Project/Area Number: 26750024
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Takasaki University of Health and Welfare
• Principal Investigator: Kumakura Kei 高崎健康福祉大学, 健康福祉学部, 助教 (80516930)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: きのこ / 酵素 / 遺伝子 / プロテアーゼ / グルカナーゼ

Outline of Final Research Achievements

This study focused on enzymes of a Ganoderma lucidum water extract. These enzymes studied were glutamic acid protease (GlGPA) and β-1,3-glucanase (GlGH128A). Full-length cDNA sequences of GlGPA and GlGH128A were obtained by using rapid amplification of cDNA ends (RACE)-PCR. Further, gene expression of GlGPA and GlGH128A was analyzed during growth stages and the harvest storage period. It was revealed that gene expression of GlGPA increased in mycelium stage, primordium of fruiting body stage, fruiting body before ripening stage, and fruiting body of 4 weeks after harvesting. On the other hand, gene expression of GlGH128A increased in fruiting bodies of 1, 2, and 4 weeks after harvesting. These results suggest that GlGPA and GlGH128A are important for morphogenesis and aging of bruiting body.

Free Research Field

食品機能学