2015 Fiscal Year Annual Research Report
Engineering thick tissues in vivo using oxygen-releasing pro-angiogenic biomaterials
| Project/Area Number |
26750144
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| Research Institution | The University of Tokyo |
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Principal Investigator |
モンターニュ ケヴィン 東京大学, 工学(系)研究科(研究院), 助教 (50606118)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | Hypoxia / Biomaterials / Tissue engineering |
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| Outline of Annual Research Achievements |
Engineering thick, cell-dense artificial tissues to replace diseased organs has been hindered so far by the difficulty in maintaining the cells alive after implantation due to insufficient oxygen supply. Recently, biomaterials that release oxygen thanks to the incorporation of various peroxides have been used successfully in vitro to supply oxygen to cultured cells, preventing cell death due to hypoxia. However, such materials have so far not been tested for the development of thick (several mm) tissues in vitro or in vivo.
The aim of this research was to create thick tissue constructs based on innovative biomaterials that both promote blood vessel growth upon implantation into a host while simultaneously releasing oxygen to maintain the viability of the construct.
So far, oxygen-releasing biomaterials have been prepared consisting of polydimethylsiloxane sponges containing up to 20% of calcium peroxide. Those sponges have been shown to increase the oxygen concentration in the cell culture medium. Furthermore, cells cultured at a high density in the sponges were still viable after up to seven days in culture and showed a decreased hypoxic response compared to cells cultured in control sponges. Animal experiments are still underway to show a beneficial effect in vivo on vascularization of the implanted constructs.
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[Presentation] 軟骨前駆細胞における高静水圧刺激によるストレス反応および脱分化メカニズムの検討2016
Author(s)
Montagne Kevin, Ogasawara Mutsuo, Furukawa Katsuko, Ushida Takashi
Organizer
28th bioengineering lecture meeting of the Japan Society of Mechanical Engineers
Place of Presentation
東京工業大学 大岡山キャンパス、東京都、目黒区
Year and Date
2016-01-09 – 2016-01-10
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