Development of microscopic single-molecule pathological examination device for DNA methylation

2017 Fiscal Year Final Research Report

• Project/Area Number: 26750146
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Nagoya University
• Principal Investigator: ONOSHIMA Daisuke 名古屋大学, 未来社会創造機構, 特任講師 (40510219)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: １分子計測(SMD) / 細胞・組織 / マイクロ・ナノデバイス

Outline of Final Research Achievements

Image analysis of methylated DNA has a great potential for exploring epigenomic change in cancer cells. In order to visualize signal variation caused by DNA methylation, it is crucial that the DNA molecules are arrayed in parallel direction inside the narrow microscopic field. Here we show that methylated DNA molecules can be trapped and stretched at the patterned grooves on the surface of microfluidic channel by flow. The demonstrated DNA aligning technique enables simultaneous molecular imaging and detection for cancer diagnosis at the single molecule level.

Free Research Field

ナノバイオデバイス工学