Development of a cytoplasmic RNA vector to modulate microRNA function for cellular reprogramming

2015 Fiscal Year Final Research Report

• Project/Area Number: 26750163
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: SANO Masayuki 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 主任研究員 (80415687)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: マイクロRNA / RNAベクター / 細胞改変技術 / 遺伝子導入 / 機能制御

Outline of Final Research Achievements

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression by binding to the 3’ unltanslated region of target mRNAs. It has been demonstrated that several tissue-specific miRNAs can modulate cellular reprogramming. In this study, we tried to construct a cytoplasmic RNA vector, based on Sendai virus, which not only produces miRNAs but also inhibits the miRNA activity, so as to apply in cellular reprogramming.

Free Research Field

複合領域