2016 Fiscal Year Final Research Report
Novel method for live cell actin imaging based on small molecule fluorescent probe
Project/Area Number |
26750369
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Chemical biology
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Research Institution | The University of Tokyo |
Principal Investigator |
Ueno Tasuku 東京大学, 大学院薬学系研究科(薬学部), 助教 (60462660)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Keywords | 蛍光プローブ / アクチン / イメージング |
Outline of Final Research Achievements |
The dynamic behavior of the actin cytoskeleton is a critical part of most cellular activities, e.g., membrane trafficking, cell movement and neurite extension. Therefore, visualization of actin cytoskeleton dynamics is essential to understand its roles in regulating cellular functions. In the present study, novel small molecule based fluorescence probe for F-actin visualization probes were successfully developed. The developed probe is simple to use, and has sufficiently high sensitivity and specificity for actin cytoskelton. It is also showed that the probe was applicable for live cell fluorescence imaging method. This would be nice piece of work a potentially important to researchers interested in the biology associated with actin dynamics.
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Free Research Field |
ケミカルバイオロジー
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