2016 Fiscal Year Final Research Report
Development of fluorescent probes for detection of histone deacetylase activity
Project/Area Number |
26750371
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Chemical biology
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Research Institution | Osaka University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Keywords | 蛍光プローブ / ヒストン脱アセチル化酵素 / DNA |
Outline of Final Research Achievements |
In this study, fluorescent probes were developed for detecting histone deacetylase (HDAC) activity involved in epigenetic gene expression. We designed and synthesized peptide conjugates between nuclear staining dye and HDAC substrates based on the control of DNA interaction upon deacetylation. Reaction of the probes with HDAC in the presence of DNA resulted in fluorescence enhancement in concomitant with the increase of DNA binding affinity. Our method demonstrated rapid detection of HDAC activity with a one-pot procedure.
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Free Research Field |
ケミカルバイオロジー
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