2016 Fiscal Year Final Research Report
Development of a method for phosphorylation signaling
| Project/Area Number |
26750372
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | リン酸化シグナリング / プロテインキナーゼ / マルチPK抗体 / Phos-tag / プロテオミクス / 二次元電気泳動 |
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| Outline of Final Research Achievements |
To analyze expression and phosphorylation state of cellular protein kinases, we developed new method using Multi-PK antibodies and Phos-tag techniques. Analysis using human promyelocytic leukemia HL-60 cells, we identified and detected protein kinase involved in resistance of anticancer agent and differentiation. Our present study revealed that the new methods can be used for the elucidating of biological phenomena and disease pathogenesis through analysis of intracellular protein kinases.
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| Free Research Field |
生化学
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