In vivo imaging of transplanted stem cells by OTN-NIR fluorescence quantum dots

2017 Fiscal Year Final Research Report

• Project/Area Number: 26790006
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nanostructural physics
• Research Institution: Nagoya University
• Principal Investigator: Yukawa Hiroshi 名古屋大学, 工学研究科, 特任講師 (30634646)
• Co-Investigator(Renkei-kenkyūsha): Baba Yoshinobu 名古屋大学, 大学院工学研究科, 教授 (30183916) ; Ishikawa Tetsuya 名古屋大学, 大学院医学系研究科, 教授 (10288508)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 量子ドット / 幹細胞 / イメージング

Outline of Final Research Achievements

Nobel Cd free quantum dots (QDs) which emit 1,000 nm wavelength fluorescence at infrared region with high compatibility were synthesized, and optical properties (fluorescence intensity, quantum yield, life time, and stability) of the QDs were checked. In addition, the transduction efficiency and cytotoxicity of the QDs to stem cells were investigated, and the self-proliferation and multipotency of stem cells labeled with QDs were checked. Moreover, in vivo fluorescence imaging of transplanted stem cells labeled with QDs were addressed and was found to be useful for the assessment of acute liver failure mice.

Free Research Field

再生医工学