2015 Fiscal Year Final Research Report
On Chip Detection of Tau Protein Using Microtubule Based Bioassay
| Project/Area Number |
26790030
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nano/Microsystems
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | Protein detection / On-chip detection / Tau protein / Microtubules / Kinesin |
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| Outline of Final Research Achievements |
This study shows that development novel tau detection method in an on-chip format. The proposed detection system is based on kinesin-coated bead motion along immobilized MTs. Chemically stabilized MTs (without tau) are used as the rail for kinesin motion. Kinesin molecules work collectively to move a bead along MTs. Kinesin molecule encountering a tau protein on the MT surface stops/detaches. Motion continues when another kinesin molecule starts moving. Such “switching” decreases bead velocity. Results could be achieved with the chip in a time and cost efficient way due to parallel processing. Moreover, a demonstration of an additional pre-incubation technique was introduced, which can solve the existing issues on detecting mutated tau. This novel technique can be used to amplify the sensitivity reaching the physiological levels. After necessary optimizations, clinical samples should be used to test the performance for demonstrating the diagnostic capabilities.
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| Free Research Field |
バイオMEMS
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