2014 Fiscal Year Research-status Report
Direct study of neurotransmitter/Action potential correlation with a CMOS integrated carbon nanotube sensors arrays
| Project/Area Number |
26790035
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
セシュピン フロラン 独立行政法人理化学研究所, 生命システム研究センター, 特別研究員 (20650641)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | Ｃａｒｂｏｎ ｎａｎｏｔｕｂｅ / biosensors / neurotransmitters / CMOS / biofunctionalization / Nanosensors |
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| Outline of Annual Research Achievements |
We have created a high-density array of carbon nanotube field effect transistor sensors (CNT-FET) and we have proved that they can be used for the sensing of neurotransmitters after functionalization with specific enzyme. More specifically, we have improved existing dielectrophoresis integration protocol to achieve yield of about 80% of small bundle integration in an array of 1024 electrodes. By using a liquid gate configuration we have proved a high dependency of the CNT to the conductance of the fabricated devices. Finally, we have use a non-covalent functionalization method to bind glutamate oxidase to the CNT-FET and we have shown that such biosensors are sensitive to the presence of glutamate. This accomplishment represent the highest number of CMOS integrated CNT-FET and have a promising potential for the targeted applications.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We are now developing the sensing of glutamate using glutamate oxidase functionalization of the CNT-FET. The first proofs of sensing have been done but improvement of the measure protocol has to be realized to detect lower concentration in complex environment. In the same time, we are also proceeding to the recording of signal from cultured neuron on top of CNT-FET sensors arrays. Statistical analyses of the recorded data are now performed to get the valuable information extraction route.
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| Strategy for Future Research Activity |
The next step of this project is the study of different functionalization of the sensors in order to achieve detection at low concentration in an environment containing several analyte. After, this first step achievement we will start a large number of recording experiment in cells culture and correlate the glutamate release to neuron activity. Finally, we will focus our experiment on different type of neurons or culture. For example culture with abnormal behaviors.
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| Causes of Carryover |
The equipment purchased was cheaper than expected.
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| Expenditure Plan for Carryover Budget |
This excess will be used for purchasing other type of analyze chemicals.
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