Exploitation of technique for precise measurement of hierarchical structure and ion-specific density distribution of biomembrane interfaces

2015 Fiscal Year Final Research Report

• Project/Area Number: 26800223
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological physics/Chemical physics/Soft matter physics
• Research Institution: Kyoto University
• Principal Investigator: Yamamoto Akihisa 京都大学, 物質-細胞統合システム拠点, 研究員 (90706805)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 生体膜 / 生物物理 / X線蛍光散乱 / X線反射率 / 静電相互作用

Outline of Final Research Achievements

Within this subject, by constructing the model of cell membranes, the effect of electronic charges contained in the membrane and proteins around membranes on membrane structure and the interaction between membrane and proteins was experimentally revealed. Irradiation of X-ray onto the membrane model generates the reflection of X-ray (XRR: X-ray reflectivity) and emission of fluorescent X-ray from atoms (GIXF: grazing incident X-ray fluorescence). Measurement of these signal intensities allowed us to determine the distribution of charges within Angstrom accuracy, which is in the order of the typical size scale of atoms.

Free Research Field

ソフトマター物理