2015 Fiscal Year Final Research Report
Renaturation of Aggregated Proteins Treated with Hydrated Ionic Liquids
| Project/Area Number |
26810066
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Polymer chemistry
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
FUJITA Kyoko 東京農工大学, 工学(系)研究科(研究院), 講師 (90447508)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | リフォールディング / イオン液体 / 水和 |
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| Outline of Final Research Achievements |
We have investigated simple method to realize dissolution and refolding of aggregated recombinant protein from E. coli in hydrated ionic liquids. Among the investigated ionic structures, a pair of cholinium cation and dihydrogen phosphate anion showed direct dissolution of the aggregated protein with subsequent formation to the folding state, when it was controlled the number of water molecules.
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| Free Research Field |
生物電気化学、生体高分子、イオン液体
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