Development of sensor arrays consisting of polyion complexes between enzymes and polyelectrolytes for markerless and noninvasive evaluation of stem cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 26810074
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Tomita Shunsuke 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (50726817)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 幹細胞 / 酵素 / 高分子電解質 / 多変量解析 / バイオセンサ / ポリイオン複合体

Outline of Final Research Achievements

We developed a library of polyion complexes between enzymes and polyelectrolytes for markerless and noninvasive evaluation of stem cells. Cationic block copolymers with various hydrophobic groups were first synthesized, and used to construct highly cross-reactive molecular libraries by the formation of complexes with anionic enzymes. Analysis of cell culture supernatants using these libraries allowed not only discrimination of different cancer cell lines but also lineage identification of osteogenic and adipogenic differentiation of human mesenchymal stem cells.

Free Research Field

分析化学