2016 Fiscal Year Final Research Report
Development of microfluidic device/inductively coupled plasma spectrometer for determination of trace elements in selected cells
| Project/Area Number |
26810081
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Kaori Shigeta (杉山香織) 国立研究開発法人産業技術総合研究所, 環境管理研究部門, 研究員 (20639744)
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| Research Collaborator |
NAKAZATO Tetsuya
TAO Hiroaki
HIRANO Ken
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 単一細胞分析 / 元素分析 / 質量分析 / 誘導結合プラズマ質量分析計 / ドロプレット試料導入 / インクジェット / マイクロ流体デバイス / メタロミクス |
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| Outline of Final Research Achievements |
Development of multi elements analysis system for single cells is required to reveal the functions of trace metal elements in biological cells. In this study, a system of cell-handling technology based on microfluid mechanics were establish, and sample introduction was developed to inject the droplets containing a single cell into inductively plasma mass spectrometer (ICP-MS). The microfluid device for transport of cells was designed, and behavior of HeLa cells transported through a flow channel was observed by a microscope. The time-resolved ICP-MS with shortest time resolution of 10 μs was developed to measure the transient signals generated by injection of a single cell. Detection of the transient signals from 10 nm scale - silver nano particle was achieved.
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| Free Research Field |
分析化学
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