Chimeric RNA Oligonucleotides with Triazole and Phosphate Linkages: Synthesis and RNA Interference

2015 Fiscal Year Final Research Report

• Project/Area Number: 26810083
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Tohoku University
• Principal Investigator: Fujino Tomoko 東北大学, 理学(系)研究科(研究院), 助教 (70463768)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: siRNA / キメラ型多量体 / トリアゾール

Outline of Final Research Achievements

Chimeric RNA oligonucleotides with an artificial triazole linker were synthesized using solution-phase click chemistry and solid-phase automated synthesis. Scalable synthesis methods for jointing units for the chimeric structure have been developed, and after click-coupling of the jointing units with triazole linkers, a series of chimeric oligonucleotides was prepared by utilizing a well-established phosphoramidite method for the elongation. The series of chimeric 21-mer oligonucleotides that possessed the triazole linker at different strands and positions allowed for a screening study of the RNA interference to clarify the preference of the triazole modifications in small-interfering RNA molecules.

Free Research Field

生物有機化学