2014 Fiscal Year Research-status Report
Enhanced Cell Evaluation by Phase Decomposition
| Project/Area Number |
26820086
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| Research Institution | Osaka University |
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Principal Investigator |
蔡 佳宏 大阪大学, 工学(系)研究科(研究院), 研究員 (20647490)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | cell deformability / red blood cell / microfluidics / lab on a chip |
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| Outline of Annual Research Achievements |
A total of five papers, including 2 SCI-indexed journal papers and 3 conference papers, are published in the last year of this research project.
The experimental platform for the proposed cell evaluation has been successfully developed, and is capable of manipulating a cell moving in a simple harmonic motion up to 130 Hz (Monzawa, Kaneko, Tsai et al. Biomicrofluidics 9(1)p014114, 2015). Microfluidic channel designs that can align cells have been developed for enhancing the evaluation system (Tsai et al. RSC Advances, 4(85)p45050-45058,2014 and IEEE-URAI2014, p522-524). The difference of mechanism between cell flow under a positive-pressure and negative-pressure driven flow is investigated (MicroTAS2014, p793-795). An on-chip pressure sensor for assisting the evaluation of cell deformability is developed (IEEE MEMS2015, p722-725).
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The plan of the first year in the proposal is to build up the experimental system and have preliminary tests on cell deformability. The current progress smoothly catches up with the plan. The hardware of the system has been set up, and the image-processing algorithm for cell tracking has been developed. The deformability of the red blood cells from a patient with Multiple Myeloma is found very different from the ones with healthy subjects. The results have been published in International Journal with impact factor of 3.7.
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| Strategy for Future Research Activity |
There are two aims for this year. The first aim is to further investigate the relation between cell deformability and human diseases. It has been scheduled for the experiments on the red blood cells from the patients with cardiovascular circulation problems. The second aim is to improving the proposed evaluation system, so that it can be more accurate on deformability evaluation and more convenient for everyone to use.
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