Evaluation of tumor penetration ability of nanoparticles using 3D cell culture system toward particle design optimization

2015 Fiscal Year Final Research Report

• Project/Area Number: 26820356
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: The University of Tokyo
• Principal Investigator: Ohta Seiichi 東京大学, 医学(系)研究科(研究院), 助教 (40723284)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ナノ粒子 / 腫瘍浸透性 / スフェロイド / ヒアルロン酸 / ナノゲル / ドラッグデリバリー

Outline of Final Research Achievements

Multicellular 3D cancer spheroid was fabricated as a tumor model using MKN74 human gastric cancer cells. Penetration behavior of model nanoparticles, hyaluronan nanogels incorporating anti-cancer drug cisplatin, into tumor spheroid was successfully visualized. It was found that the penetration ability of hyaluronan nanogels was significantly higher than that of their linear analog, suggesting the importance of the shape of drug nanocarriers. Furthermore, a mathematical model based on reaction-diffusion equation was proposed to describe the penetration behavior of nanoparticles. The model calculation fitted the experimental results well.

Free Research Field

医用化学工学