2015 Fiscal Year Final Research Report
Development of a nanostructured hybrid antibody molecule with DNA scaffold
| Project/Area Number |
26820360
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Nagoya University |
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Principal Investigator |
Kato Teruyo 名古屋大学, 生命農学研究科, 研究員 (40727640)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 学会発表 |
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| Outline of Final Research Achievements |
Antibodies have become essential tool in the life technology fields and their applications as a high-sensitivity sensor are increasing.
In this research, we have developed a novel sensor molecule named ‘Hybribody’ which consists of DNA (PCR product or plasmid), DNA-binding protein, and antibody (scFv). First, we established an efficient production scheme for fusion proteins of DNA-binding protein and scFv using E. coli expression systems with an N-teminal tag and chaperon co-expression. Bivalent antibody-DNA hybrid molecule, Hybribody, was formed by mixing with the purified proteins and DNA fragments.
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| Free Research Field |
分子生物工学
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