In vitro brain development analysis using iPS cells and genome editing technologies

2016 Fiscal Year Final Research Report

• Project/Area Number: 26830018
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Keio University
• Principal Investigator: Ishikawa Mitsuru 慶應義塾大学, 医学部(信濃町), 特任助教 (10613995)
• Research Collaborator: TANAKA Shoma
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: iPS 細胞 / ゲノム編集 / 大脳皮質 / オルガノイド / 神経発達 / 進化

Outline of Final Research Achievements

Toward estimating characteristics of human or non-human primate-specific genes or their specific CNVs, we are investigating how the annotated genes function in neuronal development using human induced pluripotent stem cells and genome editing techniques. In this study, we have developed two efficient neural induction methods from human iPS cells. One is purification of excitatory neurons in a dish, by which biochemical analysis became to well function because of its less heterogeneity. On the other hand, generating human cerebral organoid system have been developed. Using CRSPR/Cas9 to human genome, we demonstrated that knock out of a neural development and microcephaly-related gene generated abnormal construction of human cortical layers. In summary, our studies would become a support of identifying and evaluating therapeutic strategies to ameliorate the symptoms of neurodevelopmental pathogenesis.

Free Research Field

神経生物学