Revelation of the induction mechanism of plasticity during motor learning with the two-photon imaging and optical manipulation

2016 Fiscal Year Final Research Report

• Project/Area Number: 26830020
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: The University of Tokyo (2016); National Institute for Basic Biology (2014-2015)
• Principal Investigator: MASAMIZU Yoshito 東京大学, 大学院医学系研究科(医学部), 助教 (90608530)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ２光子イメージング / 2光子励起顕微鏡 / 運動学習 / アデノ随伴ウイルス / 逆行性感染

Outline of Final Research Achievements

First, fluorescence calcium sensor was expressed in only corticostriatal neurons or corticospinal neurons of mouse primary motor cortex by the retrograde gene transfer of adeno-associated virus. Next, I performed two-photon calcium imaging during a self-initiated lever-pull task for two weeks. These imaging data revealed that, during motor learning, the population which increased firing rate in corticostriatal neurons was different from that in corticospinal neurons.

Free Research Field

神経科学