Functional analysis of SHP-1 in the central nervous systems

2015 Fiscal Year Final Research Report

• Project/Area Number: 26830028
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Osaka University
• Principal Investigator: Fujita Yuki 大阪大学, 医学(系)研究科(研究院), 助教 (60631215)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 中枢神経 / SHP-1

Outline of Final Research Achievements

Protein tyrosine phosphatases regulate various signaling mechanisms in the central nervous system (CNS). Src homology 2-containing phosphatase (SHP)-1 is the classical non-receptor protein tyrosine phosphatase (PTP), and involved in axon growth inhibition and neuronal apoptosis. The longer isoform of SHP-1, SHP-1L has also been identified but its role in the CNS remains to be unclear. In this study, we show that overexpression of SHP-1L as well as SHP-1 increases neuronal apoptosis. However, overexpression of SHP-1 or SHP-1L in mouse cortical neurons does not affect neurite length. Filamin A (FLNa), which is an actin-binding cytoskeletal protein, inhibits apoptosis induced by SHP-1 and SHP-1L. Co-expression of SHP-1 along with FLNa increases cytoplasmic localization of SHP-1. Our results suggest that SHP-1 and SHP-1L negatively regulates neuronal survival via FLNa.

Free Research Field

分子神経科学