2016 Fiscal Year Final Research Report
Generation and analysis of asthenozoospermia mouse models using CRISPR/Cas9 system
| Project/Area Number |
26830056
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ゲノム編集 / 受精 / 精子 / 鞭毛 |
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| Outline of Final Research Achievements |
Of all infertility cases, half are due to male infertility and approximately 20% of male infertility is thought to be due to impaired sperm motility (asthenozoospermia). In this study, we knocked out genes that are implicated in sperm motility using the CRISPR/Cas9 system to find genes that cause asthenozoospermia. As a result, we generated 8 lines that exhibit male subfertility or sterility because of impaired sperm motility or abnormal sperm tail formation. Among these genes, we analyzed the functions of Ppp3r2 and Cabyr extensively. Analyses of other genes will be conducted subsequently.
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| Free Research Field |
生殖生理
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