2015 Fiscal Year Final Research Report
Studies on intracellular transport of cartilage matrix
| Project/Area Number |
26830059
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Nippon Veterinary and Life Science University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 軟骨形成 / 繋留因子 / 小胞輸送 / ゴルジ体 / 実験動物 / 細胞外基質 |
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| Outline of Final Research Achievements |
Giantin is vesicle tethering factor that functions at cis-Golgi compartment. Immunohistological analysis showed that giantin were expressed in various types of cells including chondrocyte. Morphology of golgi apparatus in chondrocytes lacking giantin were indistinguishable from that of normal cell by immunofluorescence of golgi marker proteins. Cartilage of embryos lacking giantin showed similar level of type II collagen and fibronectin. has markedly reduced aggrecan and link protein, and increased type XI collagen. These results suggest that tethering factors have unique and redundant roles, and that giantin play a pivotal role in secretion and/or synthesis of specific cartilage matrix.
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| Free Research Field |
動物遺伝学
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