2015 Fiscal Year Final Research Report
Functional analysis of WTAP protein complex on alternative splicing
| Project/Area Number |
26830124
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Horiuchi Keiko 東京大学, 先端科学技術研究センター, 助教 (00456203)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | RNAスプライシング / WTAP / 細胞周期 / SUV420H2 |
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| Outline of Final Research Achievements |
Wilms’ tumor 1-associating protein (WTAP) is a putative splicing regulator which is required for mouse early embryo development and cell cycle progression. We previously isolated the proteins which interact with WTAP, including VIRILIZER, CBLL1, KIAA0853, RBM15, BCLAF1, THRAP3 and general splicing regulators.To identify the alternative RNA splicing regulated by WTAP protein complex, we performed high-throughput mRNA sequencing using WTAP knockdown cells. Interestingly, WTAP regulates alternative splicing of histone H4 Lys 20 methyltransferases SUV420H1 and SUV420H2 by promoting a production of the truncated isoforms, leading to a change in the global H4K20 methylation level.
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| Free Research Field |
RNAスプライシング
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