High efficiency of human iPS cell induction by the novel DNA recognition compound to regulate the EMT

2015 Fiscal Year Final Research Report

• Project/Area Number: 26830134
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical genome science
• Research Institution: Nihon University
• Principal Investigator: SAITO Kosuke 日本大学, 医学部, 研究員 (80624163)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: EMT / iPS / PIポリアミド

Outline of Final Research Achievements

We investigated whether PI polyamides designed targeting of the TGF-β1 have the effect regulation of EMT/MET, and increase the human iPS cell induction efficiency. We designed and synthesized three different types of PI polyamides designed targeting of the TGF-β1. We were administered PI polyamide to human fibroblasts transfected with exogenous reprogramming factor by Sendai virus vector and grown in human iPS induction cultures were seeded on feeder cells, was observed a significant increase in alkaline phosphatase-positive colony number of PI polyamide treated group compared with the non-treated group, we also found that the expression of undifferentiated markers was observed in these colonies. It was found to regulate of EMT/MET, and increase the human iPS cell induction efficiency in human fibroblasts by PI polyamides targeting human TGF-beta gene promoter region.

Free Research Field

ゲノム医科学