Molecular mechanism of genome maintenance by histone H1 ubiquitination

2015 Fiscal Year Final Research Report

• Project/Area Number: 26840003
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: University of Tsukuba
• Principal Investigator: Kato Kohsuke 筑波大学, 医学医療系, 助教 (90466673)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ゲノム安定性 / ヒストンH1 / ユビキチン / クロマチン / G1-S遺伝子群 / 転写

Outline of Final Research Achievements

Aim of this research is to reveal the molecular mechanism of genome maintenance through the VprBP-mediated histone H1 ubiquitination. Depletion of VprBP caused the accumulation of DNA damage and cell cycle arrest at late S-G2 phase in cells. We found that VprBP is involved in transcription elongation of activating E2F and homologous recombination-repair genes in histone H1-dependent manner. These results raise a possibility that VprBP-mediated histone H1 ubiquitination is involved in genome maintenance through the transcriptional regulation of genes activated in cell cycle G1-S phases.

Free Research Field

分子生物学