2015 Fiscal Year Final Research Report
Elucidation of tRNA transport mechanism under heat stress
| Project/Area Number |
26840010
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 伸長tRNA / tRNA顆粒 / nuclear stress bodies / リアルタイムイメージング |
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| Outline of Final Research Achievements |
To investigate elongator tRNA (eMet) localization, we tried things written below. We revealed sequence of DNA/RNA hybrid which fluoresces by RNaseH digestion. Furthermore, molecular beacon (MB) optimized target site on eMet could detec mature eMet effectively.
Formation of tRNA granules was inhibited by knockdown of proteins forming nuclear stress bodies. Nuclear stress bodies colocalized with tRNA granules. In addition, mammalian target of rapamycin is important to nuclear accumulation of eMet.
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| Free Research Field |
生物学
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