2015 Fiscal Year Final Research Report
NMR analyses of the structural equilibrium in tyrosine kinases
| Project/Area Number |
26840014
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Keio University (2015)
Hokkaido University (2014) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | キナーゼ / NMR |
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| Outline of Final Research Achievements |
Tyrosine kinases are major targets for anticancer drug. To understand the dynamic regulation of tyrosine kinases, NMR analyses of the structural equilibrium were performed. In this study, E coli. expression system and purification protocol for c-Src, Fyn, and c-Abl were successfully established. NMR spectral changes upon phosphorylation of c-Src and upon binding to drugs were observed. NMR approach would be useful for understanding the dynamic processes in the regulation of tyrosine kinases.
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| Free Research Field |
構造生物化学
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