Structural and functional analysis of gap junction channels using electron microscopy

2015 Fiscal Year Final Research Report

• Project/Area Number: 26840019
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Nagoya University
• Principal Investigator: Oshima Atsunori 名古屋大学, 名古屋大学細胞生理学研究センター, 准教授 (80456847)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ギャップ結合チャネル / イネキシン / 電子顕微鏡 / 三次元再構成 / 二次元結晶

Outline of Final Research Achievements

In this project, the structure of C. elegans innexin-6 (INX-6) was investigated using electron crystallography. Two dimensional crystals of N-terminal deleted INX-6 where 2 to 19 were eliminated were successfully obtained. The structure of INX-6delN reveals the six-teen subunits, a hexadecamer. The plug density was observed on the channel pore pathway, suggesting a structure in a closed form. These results have been published as a research article

Free Research Field

構造生物学