Direct observation of the ligand-dissociation dynamics of the hemoglobin

2016 Fiscal Year Final Research Report

• Project/Area Number: 26840028
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Jichi Medical University (2015-2016); High Energy Accelerator Research Organization (2014)
• Principal Investigator: Sato Ayana 自治医科大学, 医学部, 助教 (50717709)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ヘモグロビン / 光解離 / 結晶構造解析 / 時間分解測定

Outline of Final Research Achievements

To trace structural changes of carbonmonoxy hemoglobin associated with ligand photolysis, the X-ray crystallography under laser irradiation conditions were carried out. Using a set of coordinates of those intermediate states of hemoglobin, the quantitative conformation change in inner subunit and intra subunits of hemoglobin were appraised. The sequential structural change in each subunit propagated across the overall tetrameric molecule and produced the quaternary conformational motion.

Free Research Field

生物物理