Single-molecule characterization of target DNA search of tumor suppressor p53

2015 Fiscal Year Final Research Report

• Project/Area Number: 26840045
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Tohoku University
• Principal Investigator: Kamagata Kiyoto 東北大学, 多元物質科学研究所, 助教 (90432492)
• Research Collaborator: SUGIYAMA HIROSHI 京都大学, 大学院理学研究科, 教授 (50183843) ; ENDO MASAYUKI 京都大学, 物質-細胞統合システム拠点, 准教授 (70335389)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 蛋白質 / DNA / １分子計測

Outline of Final Research Achievements

p53 can preventDNA. the cancer of a living cell by binding to a part of DNA (target). In this study, we investigated the mechanism in which p53 searches and binds to the target and the role of disordered region of p53. We measured the movement of p53 along DNA. We found that p53 has a low target recognition probability（TRP） and regulates TRP by posttransmodifications of p53. Furhtermore, we found that elongation of the disordered region of p53 caused the loss of specific binding to DNA.

Free Research Field

蛋白質科学、生物物理学