2015 Fiscal Year Final Research Report
Identification of key residues for control of the coupling between ATP hydrolysis and substrate transport of an ABC transporter
| Project/Area Number |
26840048
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 膜タンパク質 / ABCトランスポーター / X線結晶構造解析 / 基質輸送 / ATP |
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| Outline of Final Research Achievements |
Human ABC transporters are predicted to have a characteristic mechanism to control the substrate transport driven by hydrolysis of ATP. In this study, structural and functional analyses were performed using CmABCB1 mutant designed based on the crystal structures of CmABCB1, the function of which is similar to that of human ABC transporter. The analyses revealed that glutamine and alanine residues localized on the substrate transport pathway in the transmembrane domain of CmABCB1 act as an important role in the control mechanism.
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| Free Research Field |
構造生物学
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