2015 Fiscal Year Final Research Report
Designing a highly specific transcription factor
Project/Area Number |
26840054
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biophysics
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Research Institution | Japan Atomic Energy Agency |
Principal Investigator |
Sunami Tomoko 国立研究開発法人日本原子力研究開発機構, 原子力科学研究部門 量子ビーム応用研究センター, 研究副主幹 (50554648)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Keywords | 転写因子 / タンパク質・DNA相互作用 / Bacterial One-Hybrid法 |
Outline of Final Research Achievements |
Our purpose is to develop proteins with properties different from widely used genome-editing proteins such as TALEN and CRISPR. In this study, we rationally designed novel proteins based on engrailed homeodomain and confirmed the binding specificity using bacterial one-hybrid assay. We successfully designed molecules with a strong affinity and high specificity for the target sequence which has two engrailed homeodomain binding sites. Also, we developed a method to evaluate molecular flexibility of DNA using electron density map of X-ray crystal structures.
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Free Research Field |
分子生物学、構造生物学
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