2016 Fiscal Year Final Research Report
Single-molecule imaging analysis of oligomerization of GPCRs
Project/Area Number |
26840055
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Biophysics
|
Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Yanagawa Masataka 国立研究開発法人理化学研究所, 佐甲細胞情報研究室, 研究員 (70609792)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | GPCR / 代謝型グルタミン酸受容体 / セロトニン受容体 / 1分子イメージング / 多量体化 / FRET |
Outline of Final Research Achievements |
G protein-coupled receptors (GPCRs) are major drug targets. It has been reported that various GPCRs form dimers regulating their functions. However, a role of higher-order oligomerization of GPCRs in living cell is unclear. Here, we performed single-molecule imaging and FRET analysis of metabotropic glutamate receptor (mGluR) and serotonine receptor (5HTR) as model GPCRs. Single-molecule imaging analysis in living cell demonstrated that the fraction of the immobile higher-order oligomer of mGluRs, which is related to the recruitment of receptors into clathrin-coated pits, increased upon activation. The FRET analysis suggested that a dissociation of mGluR/5HTR hetero-oligomer occurs upon activation of mGluR.
|
Free Research Field |
生物物理学
|