2015 Fiscal Year Final Research Report
Analysis of ER-associated degradation components by multiple knockout cell lines using TALEN
| Project/Area Number |
26840065
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Kyoto University (2015)
Okazaki Research Facilities, National Institutes of Natural Sciences (2014) |
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Principal Investigator |
Ninagawa Satoshi 京都大学, 理学(系)研究科(研究院), 研究員 (80647991)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | EDEM / mannose / N-glycan / 小胞体関連分解 |
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| Outline of Final Research Achievements |
Glycoproteins misfolded in the endoplasmic reticulum (ER) are subjected to ER-associated glycoprotein degradation. In this process, N-glycan mannose trimming is required. We clearly showed that this process is initiated by EDEM2 and completed by EDEM3/EDEM1.
We also demonstrated that higher eukaryotes are able to extract severely misfolded glycoproteins from glycoprotein ERAD and target them to the non-glycoprotein ERAD pathway to maintain the homeostasis of the ER.
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| Free Research Field |
細胞生物学
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